Isis Pharmaceuticals, Inc.

Douglas G Brooks

Susan M Freier

Lex M Cowsert

Jacqueline R Wyatt

Brenda F Baker

John McNeil

Timothy A Vickers

Henri M Sasmor

Alexander H Borchers

Cara Ohashi

Interative, preferably computer based iterative processes for generating synthetic compounds with desired physical, chemical and/or bioactive properties, i.e., active compounds, are provided. During iterations of the processes, a target nucleic acid sequence is provided or selected, and a library of candidate nucleobase sequences is generated in silico according to defined criteria. A 'virtual' oligonucleotide chemistry is chosen and a library of virtual oligonucleotide compounds having the selected nucleobase sequences is generated. These virtual compounds are reviewed and compounds predicted to have particular properties are selected. The selected compounds are robotically synthesized and are preferably robotically assayed for a desired physical, chemical or biological activity. Active compounds are thus generated and, at the same time, preferred sequences and regions of the target nucleic acid that are amenable to oligonucleotide or sequence-based modulation are identified.

System of components for preparing oligonucleotides

Growing community of inventors

San Marcos, CA, United States of America

Douglas G Brooks