Zeneca Limited

Imperial Chemical Industries Limited

University of Florida Research Foundation, Incorporated

Donald Grierson

Wolfgang Walter Schuch

Colin Roger Bird

Ian George Bridges

John Anthony Ray

Ian G Bridges

Grantley W Lycett

Andrew J Hamilton

Gregory Alan Tucker

Gloria Andrews Moore

Graham Barron Seymour

Julie Elizabeth Gray

Ruper G Fray

Jeremy M Boniwell

Beatrix Blume

Lisa Naomi Hall

Alexandros Aggelis

Susan Dale Lawrence

Rupert George Fray

Michael Holdsworth

Zoi Karvouni

Pedro Pablo Gallego-Veigas

Isaac John

Sarah Louise Barton

Andrew Hamilton

Umi Kalsom Abu-Bakar

Alexandra Louise Lowe

Cornelius Barry

Lee Colin Whotton

Steve Picton

Process for the inhibition of the production of a gene product in a plant cell which comprises generating in the cell while the gene product is being expressed mRNA from recombinant DNA coding for part only of the gene product: also constructs for use in the process, and cells and plants that carry out the process. Specifically applicable to control of fruit ripening, in particular in tomatoes.

Method for modifying production of fruit ripening enzyme

Expression of genes inserted into plants by transformation is controlled by the use of a promoter selected from Aco1, Aco2 and Aco3, the sequences of which are given. The level of expression obtained by use of these promoters varies with the stage of development of the plant.

DNA constructs and plants incorporating them

DNA sequences from muskmelon (Cucumis melo) related to fruit ripening

Co-ordinated inhibition of plant gene expression

DNA encoding fruit-ripening-related proteins, DNA constructs, cells and

Tomato ripening TOM41 compositions and methods of use

The gene of the construct pTOM5 is involved in the carotenoid pathway, which produces carotenes, lutein, xanthophylls, and pigments such as lycopene. The invention proposes to modify (inhibit or promote) the synthesis of such compounds in plants using novel DNA contructs comprising a DNA sequence homologous to some or all of the gene encoded by the clone pTOM5, preceded by a plant promoter. In particular, colour of plant parts, especially fruit, may be modified. Yellow tomatoes are disclosed.

Process for modifying the production of carotenoids in plants, and DNA,

The invention relates to the clone pTOM36, comprising a gene encoding an enzyme involved in ripening related processes in tomato. Also described are DNA constructs comprising pTOM36 and a transcriptional intiation region operative in plants such that pTOM36 RNA is generated in plant cells, and tomato cells transformed with the DNA constructs.

PTOM36 constructs and tomato cells transformed therewith

DNA, DNA constructs, cells and plants derived therefrom

A method is provided for making fruit (particularly tomatoes) having increased solids content which comprises cultivating fruit-bearing plants in which expression of genes homologous to pTOM36 is at least partially inhibited. For this purpose the fruit may be transformed with DNA constructs comprising a DNA sequence homologous to some or all of the gene encoded by the clone pTOM36. The clone is adapted to generate sense or antisense RNA under control of a plant promoter.

Transformed tomato plants

DNA constructs which inhibit ethylene production in plants comprise a transcriptional initiation region operative in plants for transcribing a DNA sequence encoding an RNA sequence (which may be inverted) complementary to a substantial run of bases showing substantial homology to an mRNA encoding an enzyme involved in ethylene biosynthesis: also plant cells comprising such constructs and plants derived therefrom showing reduced ethylene production.

DNA constructs containing the gene for ACC Oxidase, cells and plants

Recombinant DNA comprises promoter and terminator base sequences respectively upstream and downstream of an inverted base sequence complementary to a substantial sequence of bases in polygalacturonase mRNA. The antisense mRNA produced thereby delays softening of fruit, in particular tomatoes.

Dicot plants containing an antisense polygalacturonase gene segment

Recombinant DNA comprises promoter and terminator base sequences respectively upstream and downstream of a base sequence corresponding to a substantial sequence of bases in pectin esterase mRNA. The mRNA produced thereby delays softening of fruit, in particular tomatoes.

Recombinant DNA containing pectin esterase gene segements

Expression of genes in transgenic plants

DNA constructs containing segments from tomato polygalacturonase and

Growing community of inventors

Loughbrough, United Kingdom

Donald Grierson