The Brigham and Women's Hospital, Inc.

The Broad Institute, Inc.

Debasish Manna

Amit Choudhary

Basudeb Maji

Bridget K Wagner

Miseon Lee

Soumyashree Ashok Gangopadhyay

The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising CRISPR enzymes associated with at least one destabilization domain (DD) and photocaged stabilization ligands with at least one photocage molecule, along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses thereof.

Methods and compositions for optochemical control of CRISPR-CAS9

The disclosure includes zinc prodrugs for targeted delivery of therapeutic, diagnostic or imaging agents to β-cells and methods of use therefor. The disclosure also includes targeted delivery of small molecules to β-cells that stabilize and activate CRISPR effector proteins comprising at least one destabilization domain, to enable CRISPR-based genome editing and transcriptional activation or repression in β-cells.

Targeted delivery to beta cells

Growing community of inventors

Boston, MA, United States of America

Debasish Manna