Amoco Corporation

Vysis, Inc.

Intellia Therapeutics, Inc.

David V Morrissey

Mark L Collins

Kevin R Kearney

Amy Madison Rhoden Smith

Christian Dombrowski

John K Eldredge

Jonathan Douglas Finn

David Gillespie

The invention is a method of detecting nucleic acids in a sample using oligonucleotide probes which are noncovalently bound to solid supports for rapid, sensitive, hybridization assays. The method involves coating the support surface with a polynucleotide and then hybridizing a specific capture probe for each analyte to the polynucleotide by way of a short tail of the complementary polynucleotide. The immobilized probes are used to capture nucleic acid targets out of complex specimens for nonisotopic detection without the need for prior cell culture or purification of the target nucleic acids. A panel of tests can be run on each specimen simultaneously, a format that conserves precious samples. The assay can be readily automated, and can be conveniently run in a manual fashion on large numbers of samples in two to three hours.

Immobilized oligonucleotide probes and uses thereof

Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

Hybridization promotion reagents

Methods and kits are described that allow the efficient capture and detection of targets employing the dA-dT and related affinity pairs from samples potentially containing large amounts of poly(rA) and/or poly(dA).

Methods and compositions for preventing interference with affinity

Growing community of inventors

Middletown, CT, United States of America

David V Morrissey