US Government As Represented by the Secretary of the Army

David Hoover

Mikeljon Nikolich

Richard Lloyd Warren

Gerhardt G Schurig

Stephen M Boyle

Nammalwar Sriranganathan

Luther E Lindler

Ted L Hadfield

John R McOulston

Live attenuated vaccines against brucellosis and infection by other diseases are described. It has been discovered that trans complementation of thewboA gene can be used to maintain an expression vector in an attenuatedhost cell in a vaccinee. Further, heterologous antigens can be expressed using thisplatform, thus effecting a multivalent vaccine againstand the disease corresponding to the heterologous antigen.

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Live Brucella vaccines and methods for preparing the live vaccines protective against brucellosis are described. The vaccines are prepared by introducing a deletion in the rfbU gene of a strain of Brucella which results in attenuation of the strain while retaining the desired immunogenicity to initiate a protective immunogenic response. Other strains with varying levels of attenuation are described.

Live vaccine against Brucellosis

Growing community of inventors

Rockville, MD, United States of America

David Hoover