Ge Healthcare Bio-Sciences Ab

Ge Healthcare Bioprocess R&d Ab

David Buckley

Anders Larsson

Hans Berg

Henrik Ihre

Anders Hagvall

Dag Lindstrom

Eva Holmgren

Philippe Busson

Maria Brännholm

Dag Lindström

Maria Amersham Biosciences Holm

Eva Amersham Biosciences Holmgren

Anders Amersham Biosciences Hagvall

Hans Amersham Biosciences Berg

Henrik Amersham Biosciences Ihre

Anders Amersham Biosciences Larsson

The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.

Method of preparing a separation matrix

The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.

Method of preparing a chromatography matrix

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Uppsala, Sweden

David Buckley