Dsm IP Assets B.v.

Daniel Veroeijen

Albert Schaap

An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80&#xb0; C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5&#xb0; C./minute and during cooling is at least &#x2212;0.5&#xb0; C./minute. The plateau maximum temperature is from 70 to 85&#xb0; C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, &#xb0; C.) graph, one obtains a trapezium having an area less than 13,000&#xb0; C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidised) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

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Delft, Netherlands

Daniel Veroeijen