Sutro Biopharma, Inc.

Daniel Groff

Jeffrey Hanson

This disclosure provides anstrain, which lacks thioredoxin reductase activity encoded by trxB and thioredoxin 1 activity encoded by trxA, and glutathione reductase activity encoded by gor. Saidstrain expresses a mutated AhpC protein having glutathione reductase activity and a cytosolic prokaryotic disulfide isomerase. Thestrain has an oxidative cytosol and can be used to efficiently produce proteins having disulfide bonds.

strains having an oxidative cytoplasm

This disclosure provides a method of producing full-length antibodies inwith a yield of at least about 200 mg/L. Thecells typically have an oxidative cytoplasm, which is helpful for maintaining the three-dimensional structure and stability of proteins having disulfide bonds. In some cases, the molar ratio of the produced HC and LC amount from culturing theranges from about 1:1 to about 1:3 (e.g., from about 1:1 to about 1:2.5, from about 1:1 to about 1:2).

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Alameda, CA, United States of America

Daniel Groff