Sartorius Bioanalytical Instruments, Inc.

National Research Council of Canada

Essen Instruments, Inc.

Daniel Appledorn

Kirk S Schroeder

Eric William Endsley

Keith J Morton

Nevine Holtz

Bradley Neagle

Brad Neagle

David Rock

Cicely Schramm

Grigory Filonov

Eric Endsley

Bradley D Neagle

Keith Morton

Disclosed herein are ATP biosensor fusion proteins and their use for assaying ATP levels in cells.

Methods and compositions for live cell analysis of intracellular ATP

Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a 'range image' by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).

Live cell visualization and analysis

Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.

Methods and apparatus for perfusion and environment control of microplate lab ware

Methods and apparatus for perfusion and environment control of microplate labware

Growing community of inventors

Ann Arbor, MI, United States of America

Daniel Appledorn