Japan Science and Technology Agency

Tokyo Institute of Technology

Celagix Research Ltd.

Daisuke Kiga

Shigeyuki Yokoyama

Ichiro Hirao

Kensaku Sakamoto

Masahiko Uchiyama

There is provided a process for industrial production of non-naturally occurring proteins composed of less than 20 amino acids, wherein the proteins retain their original functions while being capable of site-specific modification or immobilization, or having new functions not found in nature in addition to the original functions of the proteins. Specifically there are provided a process for producing a functional non-naturally occurring protein having a specific amino acid type(s) replaced with a natural amino acid(s) other than the amino acid type(s).

Process for producing functional non-naturally occurring proteins, and method for site-specific modification and immobilization of the proteins

Tyrosyl-tRNA synthetases having a modified amino acid sequence whereby unnatural amino acids can be more efficiently incorporated than original natural amino acids. More specifically, tyrosyl-tRNA synthetases having been modified at the amino acid(s) at the 37- and/or 195-positions. A method of modifying tyrosyl-tRNA synthetase characterized in that the position of an amino acid to be modified is determined based on the 3-D structure of a tyrosyl-AMP and of tyrosyl synthetase complex and an amino acid to which this enzyme binds.

Tyrosyl-tRNA synthetase variants

Growing community of inventors

Yokohama, Japan

Daisuke Kiga