Stratos Genomics, Inc.

Zymogenetics, Inc.

Roche Sequencing Solutions, Inc.

Craig Ostrander

Mark Stamatios Kokoris

Melud Nabavi

Marc Prindle

Jack Chase

Robert Busam

Michael Kovarik

Miranda Lahman

Samantha Vellucci

Taylor Lehmann

Steven D Levin

Katherine E Lewis

Margaret Dow Moore

David W Taft

Robert J Rosler

Anitra Wolf

Megan M Lantry

Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

DPO4 polymerase variants

DP04 polymerase variants

Compositions and methods relating to soluble dimeric proteins are disclosed. The dimeric proteins comprise first and second polypeptide fusions linked via a dimerizing domain, each polypeptide fusion comprising first and second monomer domains corresponding to a cytokine or an extracellular domain of a cell-surface receptor. The monomer domains may be positioned amino terminal and carboxyl terminal to the dimerizing domain. Alternatively, the monomer domains may be positioned in tandem, either carboxyl terminal or amino terminal to the dimerizing domain. The dimeric proteins are useful in methods for therapy, diagnosis, and research.

Dimeric fusion proteins and related compositions and methods

Growing community of inventors

Bothell, WA, United States of America

Craig Ostrander