Other

Synthetic Genomics, Inc.

Codex Dna, Inc.

Clyde A Hutchison, Iii

Hamilton O Smith

Mark D Adams

Claire M Fraser

J Craig Venter

Jeannine D Gocayne

Owen White

Michael G Montague

Methods and apparatus are disclosed herein for encoding human readable text conveying a non-genetic message into nucleic acid sequences with a substantially reduced probability of biological impact and decoding such text from nucleic acid sequences. In one embodiment, each symbol of a symbol set of human readable symbols uniquely maps to a respective codon identifier. Mapping may ensure that each symbol will not map to a codon identifier that generates an amino acid residue which has a single-letter abbreviation that is the equivalent to the respective symbol. Synthetic nucleic acid sequences comprising such human readable text, and recombinant or synthetic cells comprising such sequences are provided, as well as methods of identifying cells, organisms, or samples containing such sequences.

Encoding text into nucleic acid sequences

The present invention relates, e.g., to a method for amplifying a small number of copies (e.g. a single copy) of a single-stranded circular DNA molecule (e.g. having a size of about 5-6 kb) by an isothermal rolling circle mechanism, using random or partially random primers and a F29-type DNA polymerase. The method, which can also be used for amplifying DNAs by non-rolling types of multiple displacement amplification, comprises incubating the reaction components in a small volume, e.g. about 10 &#x3bc;l or less, such as about 0.6 &#x3bc;l or less. The degree of amplification can be about 109 fold, or higher. A method for cell-free cloning of DNA, using the rolling circle amplification method of the invention, is described.

Amplification and cloning of single DNA molecules using rolling circle amplification

The present invention provides the nucleotide sequence of the entire genome of , SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies protein encoding fragments of the genome, and identifies, by position relative to two (2) genes known to flank the origin of replication, any regulatory elements which modulate the expression of the protein encoding fragments of the genome.

Nucleotide sequence of the mycoplasma genitalium genome, fragments thereof, and uses thereof

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San Diego, CA, United States of America

Clyde A Hutchison, Iii