University of Health Sciences/the Chicago Medical School

Clarke J Halfman

Fluorescence ligand binding assay of a sample containing an unknown amount of ligand is performed by making direct intensity measurements. In an immunoassay the sample is in a solution containing dye labeled analyte and an antibody specific to the analyte. Surfactant, added to the solution in an amount sufficient to form micelles, provides markedly different fluorescent intensity from bound and unbound labeled analyte if the surfactant ions and dye ions have the same charge polarity and the analyte moeity has the opposite charge polarity.

Fluorescence ligand binding assay using charge-matched dye and solvent

Fluorescence ligand binding assay of a sample containing an unknown amount of ligand may be performed by making direct intensity measurements. In an immunoassay, for example, the sample may be added to a solution containing fluorescein labeled analyte and then is added an antibody specific to the analyte. Sodium dodecyl sulfate, a surfactant, added to the solution in an amount sufficient to form micelles provides markedly different fluorescent intensity from bound and unbound labeled analyte.

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Clarke J Halfman