Food Industry Research and Development Institute

Chung-Tsai Lee

Gwo-Fang Yuan

Li-Ling Liaw

Ing-Er Hwang

Ching-Ping Tseng

Yi-Pei Chen

Chun-Lin Wang

Wen-Jung Wu

Jyh-Wei Chen

Hasiao Chi Peng

Promoters and usage thereof. Two promoters, acuF and hsp promoters, comprise the nucleotide sequences of SEQ ID NO: 1 and 2, respectively.

Promoters and usage thereof

Cholesterol inhibitor produced by, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.

Monacolin K biosynthesis genes

Growing community of inventors

Changhua County, Taiwan

Chung-Tsai Lee