Sigma-aldrich Co. LLC

Chuan Li

Brian W Ward

Keming Song

Stephanie Uder

Ron Hernan

Leanne M Snyder

Kristen E Bettinger

Kristen E Opper

Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.

Processes for directionally ligating double stranded nucleic acids

Recombinant DNA processes using a dNTP mixture containing modified nucleotides

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St. Louis, MO, United States of America

Chuan Li