Roche Molecular Systems, Inc.

Hoffmann-la Roche Inc.

F. Hoffmann-la Roche Ag

Christopher Lim Sigua

Thomas W Myers

David H Gelfand

Fred Lawrence Reichert

Lisa Vivian Kalman

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.

Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescent dyes

Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes

Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.

Reagents and methods for coupled high temperature reverse transcription

Methods for coupled high temperatures reverse transcription and

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Antioch, CA, United States of America

Christopher Lim Sigua