Degussa Aktiengesellschaft

Evonik Degussa Gmbh

Degussa Gmbh

Christine Bastuck

Georg Thierbach

Brigitte Bathe

Thomas Hermann

Mechthild Rieping

Bettina Moeckel

Nicole Dusch

Walter Pfefferle

Mike Farwick

Bettina Möckel

Michael Buchholz

The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' open reading frames of yjfA and ytfP are individually or jointly inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the yjfA and ytfP gene region; (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids; and (c) isolation of the L-amino acids.

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The invention relates to an isolated polynucleotide fromhaving a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Nucleotide sequences coding for the sigM gene

The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out:

Fermentation process for the preparation of L-amino acids using strains of the family Enterobacteriaceae

The present invention relates to polynucleotides corresponding to the nadA gene and which encode the quinolinate synthetase A protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having quinolinate synthetase A activity.

Polynucleotides encoding the nadA gene and methods of producing nicotinic acid or nicotinic acid derivatives

The present invention relates to polynucleotides corresponding to the nadC gene and which encode the nicotinate nucleotide pyrophosphorylase protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having nicotinate nucleotide pyrophosphorylase activity.

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Bielefeld, Germany

Christine Bastuck