Cornell University

Nestec S.a.

Chenwei Lin

Vincent Petiard

Steven D Tanksley

James Gérard McCarthy

James Gèrard McCarthy

Isabelle Muguette Privat

Maud Lepelley

Gerald Cheminade

Mohamed Ben Amor

Andrew Simkin

James Gerard McCarthy

Victoria Caillet

Steve Tanksley

James Gerard Mccarthy

Disclosed herein are nucleic acid molecules isolated from coffee (spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, sucrose synthase, sucrose phosphate synthase and sucrose phosphatase enzymes and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.

Nucleic acids and proteins associated with sucrose accumulation in coffee

The present invention relates to isolated polynucleotides encoding cysteine proteinases; cysteine proteinase inhibitors; and aspartic endoproteinases. The invention also relates to a transformed host cell, preferably a plant cell, in which over- or under-expression of these polynucleotides result in altered levels of coffee flavor precursor levels, specifically, amino group-containing molecules such as amino acids, peptides and proteins, in green coffee grains.

Polynucleotide encoding a cysteine protease

Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.

Dehydrin genes and promoters from coffee

Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for phenylpropanoids and flavonoids in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.

Polynucleotides encoding phenylpropanoid and flavonoid biosynthetic pathway enzymes in coffee

Disclosed herein are nucleic acid molecules isolated from coffee (spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, three types of invertase and four invertase inhibitors and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.

Nucleic acids and proteins associated with sucrose degradation in coffee

Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for carotenoids and apocarotenoids in the coffee plant are disclosed. Also disclosed are a promoter sequence from a coffee carotenoid gene, and methods for using these polynucleotides, polypeptides, and promoter sequences for gene regulation and the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation of photosynthesis in the coffee plant.

Polynucleotides encoding carotenoid and apocartenoid biosynthetic pathway enzymes in coffee

Disclosed herein are nucleic acid molecules isolated from coffee (spp.) comprising sequences that encode mannan synthase or galactomannan galactosyltransferase. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the polysaccharide molecules of coffee plants, to influence extraction characteristics and other features of coffee beans.

Nucleic acids and proteins associated with galactomannan synthesis in coffee

Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignins in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.

Polynucleotides encoding lignin biosynthetic pathway enzymes in coffee

Oleosin- and steroleosin-encoding polynucleotides from coffee plants are disclosed. Also disclosed are promoter sequences from coffee oleosin genes, and methods for using these polynucleotides and promoters for gene regulation and manipulation of flavor, aroma and other features of coffee beans.

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Menlo Park, CA, United States of America

Chenwei Lin