Peking University

Beijing Vitalstar Biotechnology Co., Ltd.

Beihao Stem Cell and Regenerative Medicine Translational Research Institute

Chengang Xiang

Jun Xu

Jinlin Wang

Yan Shi

Hongkui Deng

Ming Yin

Yuanyuan Du

Jun Jia

Nan Song

A method for inducing reprogramming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.

Methods for reprograming non-hepatocyte cells into hepatocyte cells

Growing community of inventors

Beijing, China

Chengang Xiang