Waters Technologies Corporation

Millipore Corporation

Charles Phoebe

Sylvain Gilles Cormier

Theodore A Dourdeville

John Charkoudian

David Wang

Malcolm G Pluskal

Rolf Grigat

Aaron Phoebe

Hillary Hewitson

A chromatographic interface for sample handling and manipulation between separations or analyses is provided. The interface has an interfacing unit, a sample acquisition unit, and a sample trapping unit. The sample acquisition unit includes an inflow selector valve, an outflow selector valve, and one or more collection loops. The sample trapping unit includes a trap in fluid communication with the loop and having a stationary phase. The stationary phase can trap an analyte of interest from a fraction of the chromatographic sample separation flow. The tapping unit also has a scavenging gas source and a vacuum source; both can be used to substantially dry the stationary phase.

Chromatographic interface

A method for covalent immobilization of a carbohydrate molecule with an oppositely charged surface, comprising the steps of adsorbing the carbohydrate molecule to the oppositely charged surface in proximity to reactive moieties bound to the charged surface; and next activating the bound moiety sufficiently for covalent attachment to the adsorbed carbohydrate molecule. Also disclosed is a hydrophobic microporous polymer membrane coated with a cross-linked, cationic polymer having fixed charges thereon, the coating having enhanced epoxide content for covalent immobilization of the oppositely-charged carbohydrate molecule. A novel method for sequencing carbohydrates by covalent attachment of a carbohydrate conjugate to the membrane, and subsequent treatment with glycosidases, is also presented.

Method and apparatus for covalent immobilization of charge- conjugated

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Uxbridge, MA, United States of America

Charles Phoebe