Monsanto Company

Charles Lewis, Jr

William R Tolbert

James M Schuck

Joseph Feder

Mau-Jung Kuo

Jitka V Olander

Robert E Miller

A method and apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product is disclosed. The cells are retained within a reactor vessel chamber in a semi-rigid matrix having interstices for passage of fluid nutrient medium. Fresh nutrient medium is supplied by perfusion into the matrix through relatively low porosity tubes which are suspended in the reactor chamber and which substantially traverse the matrix; expended medium and cell product is withdrawn through relatively high porosity tubes which also are suspended in the reactor chamber and which substantially traverse the matrix; and oxygenated gaseous medium is supplied by perfusion into the matrix through a selectively-permeable membrane disposed in the reactor chamber.

Static cell culture maintenance system

Monoclonal antibodies are isolated from spent culture medium of the in vitro growth of hybridoma cells by treating said spent cell culture medium or a concentrate thereof with a water-insoluble, cross-linked polyelectrolyte copolymer.

Method of isolating monoclonal antibodies from hybridoma cultures

A method of submerged cell culture is provided which employs a microcarrier support comprising cross-linked polystyrene resin beads derivatized with amino acids, peptides, or hydroxy carboxylic acids.

Method of culturing microcarrier supported cells

A serum albumin fraction is prepared in high yield and purity from plasma and other albumin-containing blood protein fractions by contacting with a resinous polymeric material having a high capacity for adsorption of albumin, heating at 65.degree. C-72.degree. C for about 1-4 hours while maintaining a pH of 5.0-5.5, and then selectively eluting the albumin from the resin-protein mixture at a pH of 3.5-4.5. The resinous polymeric materials are water-insoluble, cross-linked polyelectrolyte copolymers of ethylene and maleic anhydride containing pendant dimethylaminopropyl functional groups.

Production of serum albumin

A serum albumin fraction is prepared in high yield and purity from plasma and other albumin-containing blood protein fractions by contacting with a resinous polymeric material having a high capacity for adsorption of albumin, heating at 65.degree.-72.degree. C for about 1-4 hours while maintaining a pH of 5.0-5.5, and then selectively eluting the albumin from the resin-protein mixture at a pH of 3.5-4.5. The resinous polymeric materials are diloweralkylaminoloweralkyl-substituted cross-linked dextran polymers.

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Hazelwood, MO, United States of America

Charles Lewis, Jr