Institut Francais Du Pe'trole

Institut Fran.cedilla.ais Du Petrole

Caroline Sulzer

Remy Marchal

Jeannine Lemal

Anne-Marie Davila

A process for the fed batch production of a sophorolipid composition is described, in which culturing takes place of at least one Candida bombicola strain in a culture medium incorporating a sugar and a nitrogen source and said cultured strain is exposed in a reaction zone to a supply of an appropriate substrate under adequate aeration, temperature and pH conditions and at least once the following sequence is performed: a) the strain is continuously supplied with substrate at a supply rate in the reaction zone between 0.01 and 4 grams per hour and per liter of initial reaction volume and for a supply time such that the residual substrate concentration in the reaction zone is maintained at a value at the most equal to 18 grams per liter of initial reaction volume during said supply time; and b) the sophorolipid composition produced is recovered; the process being characterized in that the substrate essentially consists of at least one animal oil, at least one vegetable and/or at least one ester of said oil, said oils and said ester incorporating an aliphatic linear chain with 10 to 24 carbon atoms.

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Rueil Malmaison, France

Caroline Sulzer