National Research Council of Canada

Other

Carlos B Miguez

Bernard Massie

Young-Jun Choi

Alaka Mullick

Young Jun Choi

An expression system for transforming E coll with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E coll host cells may be transformed with the expression system. A method of producing a protein involves transforming an E coll host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.

Inducible/regulated gene expression system in E coli

Methylotrophic or methanotrophic bacteria such asare transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator frommay serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

The integration of genes into methylobacterium, such asATCC is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (P). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for

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Beaconsfield, Canada

Carlos B Miguez