Regeneron Pharmaceuticals, Inc.

Caitlin L Goshert

Andrew J Murphy

Lynn Macdonald

David M Valenzuela

John R McWhirter

Ka-Man Venus Lai

Jose F Rojas

Gregg S Warshaw

Corey Momont

Chris Schoenherr

Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

Nuclease-mediated DNA assembly

Growing community of inventors

Boston, MA, United States of America

Caitlin L Goshert