Ambico, Inc.

C Joseph Welter

Mark W Welter

David M Chambers

Gerald R Fitzgerald

Douglas P Quick

Lisa M Welter

Ann M Steger

The invention relates to a method for making an inactivated vaccine of Mycoplasma hyopneumoniae by inactivating the bacteria with Thimerosal. The resulting bacterin is mixed with an adjuvant of aluminum hydroxide and DEAE dextran and injected into pigs. The resulting bacterin and adjuvant mixture can also be mixed with other bacteria such as Bordetella and Pasteurella, for further adjuvant effect. Protective immunity against mycoplasmal pneumonia is elicited in swine using these vaccines.

Inactivated Mycolplasma hyopneumoniae and uses therefor

Isospora suis is propagated using a swine testicular cell line which facilitates production of sporozoites and merozoites, either or both of which can be used in a vaccine for swine. Antibodies against Isospora suis sporozoites, which inhibit the infectivity of the sporozoites for the swine testicular cells, were made. These neutralizing antibodies were used to identify an apparent sporozoite attachment protein and cloned cDNA encoding a portion of the protein. The cloned cDNA was sequenced and the corresponding amino acid sequence of the antigenic sporozoite protein revealed two areas having repeated amino acid sequences, which may likewise be used in a vaccine.

Isospora suis sporozoite antigen

The invention relates to a method for making an inactivated vaccine of Mycoplasma hyopneumoniae by inactivating the bacteria with Thimerosal. The resulting bacterin is mixed with an adjuvant of aluminum hydroxide and DEAE dextran and injected into pigs. The resulting bacterin and adjuvant mixture can also be mixed with other bacteria such as Borderella and Pasteurella, for further adjuvant effect. Protective immunity against mycoplasmal pneumonia is elicited in swine using these vaccines.

Thimerosal inactivated mycoplasma hyopneumoniae vaccine

Type-C rotavirus are propagated in swine testicular cells with reduced concentrations of proteolytic enzyme for subsequent production of antigen and antiserum for use in diagnostic kits and for killed vaccines to prevent Type C rotavirus infections. Propagation of Type C rotavirus in the ST cells can also lead to virus modification for subsequent use as a modified live virus vaccine to prevent Type C rotavirus infections.

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Des Moines, IA, United States of America

C Joseph Welter