Danisco US Inc.

The Goodyear & Tire Rubber Company

Genencor International, Inc.

Bruce A Strohm

Jayarama K Shetty

Gopal K Chotani

Michael Jay Pepsin

Nigel S Dunn-Coleman

Benjamin S Bower

Suzanne E Lantz

Toby M Baldwin

Oreste J Lantero, Jr

Vivek Sharma

Sung Ho Lee

Gang Duan

Ying Qian

Hongxian Xu

Oreste J Lantero

William Albert Cuevas

Anthony R Calabria

Paula Johanna Maria Teunissen

Floor K Kooy

Pauline Johanna Maria Teunissen

Martien H Bergsma

Collette M Blake

Douglas Ko

Matt Reboli

Eric C Singley

William A Cuevas

The present invention relates to filamentous fungal host cells and particularlyhost cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch sustrates

Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids

Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Production of isoprenoids under neutral pH conditions

[object Object]

Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates

Growing community of inventors

Beloit, WI, United States of America

Bruce A Strohm