Lallemand Hungary Liquidity Management LLC

Danstar Ferment Ag

Brooks Henningsen

Allan C Froehlich

Rintze M Zelle

Sean Covalla

Ekkarat Phrommao

James L Steele

Aaron Argyros

Michelle Oeser

Janet Fisher

Fernanda Cristina Firmino

Jeffery R Broadbent

Charles F Rice

Erin Wiswall

Ryan Skinner

Kevin S Wenger

J Kevin Kraus

Johannes Van Eijk

Rintze Zelle

Allan Froehlich

The present disclosure concerns a recombinant bacterial host cell capable of providing a nitrogen source to a yeast during fermentation to make ethanol. The recombinant bacterial host cell is capable of converting a biomass into ethanol. The recombinant bacterial host cell has at least one first genetic modification. The at least one genetic modifications confers to the recombinant bacterial host cell the ability to increase, when compared to a corresponding control bacterial cell lacking the at least one first genetic modification, the proteolytic activity associated with the recombinant bacterial host cell. The at least one genetic modification also confers the recombinant bacterial host cell the ability to provide a nitrogen source to a yeast capable of converting the biomass into ethanol, wherein the nitrogen source comprises a peptide, an amino acid and/or ammonia.

Bacterial-derived nitrogen source for ethanol fermentation

The present disclosure provides an antimicrobial composition as well as a process using same to limit microbial activity in a yeast medium. The antimicrobial composition includes at least one weak acid, optionally in combination with an acid stable bacteriocin and/or an antibiotic. The antimicrobial composition can be used for propagating yeasts and for making a fermentation product. The present disclosure also provides yeasts and lactic acid bacteria that can be used with the antimicrobial composition.

Process for reducing the activity of microbial contamination in a yeast medium

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Cell-associated heterologous food and/or feed enzymes

The present disclosure concerns a co-culture of bacterial cells for making a fermented product from a biomass. The co-culture comprising a first recombinant lactic acid bacteria (LAB) cell expressing at least one bacteriocin and a second recombinant lactic acid bacteria (LAB) cell capable of converting, at least in part, the biomass into the fermented product. The second recombinant LAB cell is immune to the bacteriocin produced by the first recombinant LAB cell. The co-culture can be used, optionally in combination with a yeast host cell, to make a fermented product. The present disclosure also provides processes for making the fermented product by using the co-culture as wells kits and media comprising the co-culture.

Bacterial cocultures expressing a bacteriocin system

Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.

Acetate toxicity tolerance in recombinant microbial host cells

The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol).

Regulation of the RAS/cAMP/PKA signaling pathway in yeasts for obtaining a fermentation product during fermentation

There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Mutations in iron-sulfur cluster proteins that improve xylose utilization

There is provided chimeric polypeptides capable of converting xylose to xylulose, engineered host cells that express the chimeric polypeptides, methods of creating chimeric polypeptides, and methods of fermenting cellulosic biomass to produce biofuels, including ethanol.

Growing community of inventors

Lebanon, NH, United States of America

Brooks Henningsen