Sangamo Biosciences, Inc.

Brian Johnstone

Fyodor Urnov

Dmitry Y Guschin

Trevor Collingwood

Alan P Wolffe

Xiao-Yong Li

Methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin, are provided.

Screening methods based on isolating a collection of polynucleotides corresponding to accessible regions of chromatin

Methods for the identification of a drug target, wherein the drug target is a component of a signal transduction pathway, are provided. The methods are based, in part, on selection of a gene of therapeutic interest and determination of regulatory DNA sequences which regulate expression of the gene in a cell of interest, followed by reconstruction of signal transduction pathways which act on molecules which bind to the regulatory sequence. Also provided are methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin.

Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions

Methods for the design of exogenous regulatory molecules, comprising a DNA-binding domain and a functional domain, are provided. The methods rely on the identification, isolation and characterization of regulatory DNA sequences obtained on the basis of their accessibility in cellular chromatin.

Growing community of inventors

Benicia, CA, United States of America

Brian Johnstone