E.i. Dupont De Nemours and Company

Danisco US Inc.

Sustainable Technology Corporation

Brian G Lefebvre

Maria C Leana

William Dean Hitz

Colin Mitchinson

Perry G Caimi

Tanja Maria Gruber

George C Fox

Tom Tao Huang

Yixin Ren

Lynn Goodfellow Broderick

Amanda Kathleen Iverson

Described are methods for increasing the production of small molecules in a submergedculture by supplementinggrowth medium with the non-enzymatic fraction of spentfermentation broth.

Method for increasing the production of small molecules in submergedculture

Though chlorine dioxide is generally used to control bacterial contamination, a method was developed which allows the use of stabilized chlorine dioxide (SCD) for controlling contamination during fermentation that uses the bacteriaas the biocatalyst, even thoughis sensitive to chlorine dioxide. Parameters were identified for inoculating a composition for fermentation withcells after a time period has elapsed following SCD addition.

Stabilized chlorine dioxide for contamination control in zymomonas fermentation

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Contamination was controlled in fermentations usingas the biocatalyst, without negative impact on fermentation production, by the addition of hop acids. The effective concentration of hop acids was found to be dependent upon the type of fermentation medium used.

Contamination was controlled in fermentations usingas the biocatalyst, without negative impact on fermentation production, by the addition of virginiamycin. The effective concentration of virginiamycin was found to be dependent upon the type of fermentation medium used.

Methods are disclosed for the production of high concentrations of ethanol from biomass usingas the ethanologen.is grown under conditions of low impeller agitation with high concentration of insoluble solids in a saccharification-fermentation mixture during a simultaneous saccharification and fermentation reaction for the production of high concentrations of ethanol.

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Wilmington, DE, United States of America

Brian G Lefebvre