The Henry M. Jackson Foundation for the Advancement of Military Medicine Inc.

National Jewish Medical and Research Center

Brian C Schaefer

John W Kappler

Philippa Marrack

Chelsi Beauregard

Vectors and methods are disclosed for immortalizing mammalian cells by co-expression of v-raf and v-myc proteins. A replication-defective viral vector is used for improved safety. The vector comprises an optional marker gene, and is especially useful for producing an immortalized macrophage by a method that involves contacting the vector with a monocyte, proliferatively growing the monocyte, growing the monocytic cell on a solid surface, and then growing the monocytic cell on a porous surface. An immortalized macrophage is also disclosed.

Immortalization of splenic and peripheral blood macrophages using a multi-cistronic v-raf/v-myc lentivirus

Disclosed are methods for evaluating the activation of Bcl10 in a cell in response to a putative stimulus, as well as methods for evaluating or identifying a regulatory compound which regulates activation of Bcl10-mediated signal transduction. These methods utilize the discovery of the activation-dependent formation in a cell of Bcl10 aggregates in a cell.

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Columbia, MD, United States of America

Brian C Schaefer