Institut National De La Recherche Agronomique

Centre National De La Recherche Scientifique

Vivalis

Ecole Normale Superieure De Lyon

Valneva

Ecole Normale Superieur De Lyon

Ens-ecole Normale Superieure De Lyon

Gourmaud Selection

Bertrand Pain

Jacques Samarut

Fabienne Guehenneux

Jean-François Vautherot

Caroline Denesvre

Laetitia Fragnet-Trapp

Fabrice Lavial

Christian Jean

Benoît Gourmaud

Bernard Alletru

Laetitia Fragnet - Trapp

Guillaume Montillet

Elodie Bachelard

Hervé Acloque

Valérie Risson

Anne-Marie Birot

The present invention relates to a method for obtaining an untransformed avian cell line enabling in vitro avian virus replication. Said method includes the following steps: a) culturing avian embryonic stem cells in the presence of a stroma for at least 3 days; b) culturing for at least 2 days in a medium having a low serum concentration; c) culturing for at least 2 days in a medium having a low serum concentration containing 1 to 10 mM of hexamethyleme bisacetamide (HMBA); d) culturing for at least 10 days in a medium having a low serum concentration; and e) culturing or freezing an avian cell line enabling avian virus replication. The invention also relates to the resulting cell line and to the use thereof in vaccine preparations.

Method for selecting a permissive cell line for replicating avian viruses

The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.

Method of making an avian cell line

The present invention relates to a method for replicating poxviruses such as vaccinia virus comprising the steps of inoculating avian embryonic stem cells with viral particles and culturing said cells in a basal medium until cells lysis occurs and newly produced viral particles are released in said medium.

Production of poxviruses with adherent or non adherent avian cell lines

The present invention relates to a method for replicating pox viruses such as vaccinia virus comprising the steps of inoculating avian embryonic stem cells with viral particles and culturing said cells in a basal medium until cells lysis occurs and newly produced viral particles are released in said medium.

A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements include insulin-like growth factors and stem cell growth factors. The medium is substantially free of active retinoic acid. A method of culturing avian embryonic cells and the resulting products are disclosed.

Active retinoic acid-free culture medium for avian totipotent embryonic stem cells

Active retinoic acid-free culture medium for chicken embryonic stem cells

Growing community of inventors

Lyons, France

Bertrand Pain