Degussa Aktiengesellschaft

Forschungszentrum Julich Gmbh

Degussa - Huls Ag

Bernd Eikmanns

Hermann Sahm

Lothar Eggeling

Yvonne Tilg

Walter Pfefferle

Bettina Mockel

Bettina M&ouml;ckel

Petra Peters-Wendisch

A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.

Nucleic acid encoding pyruvate carboxylase from coryneform glutamicum

The invention provides a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which the subunit carrying the biotin-carboxyl carrier protein domain and the biotin-carboxylase domain of the nucleotide sequence encoding the enzyme acetyl-CoA carboxylase (accBC gene) is amplified, in particular is overexpressed.

Process for the fermentative preparation of L-amino acids using coryneform bacteria

The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.

Process for the preparation of L-amino acids by fermentation and nucleotide sequences coding for the accDA gene

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Ulm, Germany

Bernd Eikmanns