Janssen Biotech, Inc.

Centocor, Inc.

Bernard Amegadzie

David M Knight

Jinquan Luo

Raymond W Sweet

Mark Hilary Cunningham

Juan C Almagro

John Wheeler

Ping Tsui

Mark Tornetta

Brian Whitaker

Haimanti Dorai

Jin Lu

Bethany Swencki-Underwood

Qiang Chen

Jeffrey Luo

Juliane Mills

Lei Shi

Lionella Borozdina-Birch

Ramachandra Reddy

Mark R Cunningham

Described and claimed herein are combinatorial synthetic Fab libraries displayed on a phage pIX protein. The libraries were built on scaffolds representing the most frequently used genes in human antibodies, which were diversified to mirror the variability of natural antibodies. After selection using a diverse panel of proteins, numerous specific and high-affinity Fabs were isolated. By a process called in-line maturation the affinity of some antibodies was improved up to one hundred-fold yielding low pM binders suitable for in vivo use. This work thus demonstrates the feasibility of displaying complex Fab libraries as pIX-fusion proteins for antibody discovery and lays the foundations for studies on the structure-function relationship of antibodies.

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Human Bone Morphogenic Protein 7 (hBMP-7) variant peptide chains, nucleic acids encoding these peptide chains, and methods of making and using the foregoing are disclosed.

BMP-7 variant compositions, methods and uses

Growing community of inventors

Phoenixville, PA, United States of America

Bernard Amegadzie