Applied Biosystems, LLC

Lynx Therapeutics, Inc.

B John Bergot

Wojciech J Stec

Bernard L Hirschbein

Bogdan Uznanski

Steven Menchen

Linda G Lee

Sam L Woo

Steven Fung

Karen L Fearon

Charles R Connell

Vergine Chakerian

N Davis Hershey

J Scott Eadie

Karen L Fearson

Chromatographic separation of phosphorothioate oligonucleotides

A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.

Spectrally resolvable rhodamine dyes for nucleic acid sequence

A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosophoramidite and/or phosphorthioamidite intermediates.

Method of synthesizing sulfurized oligonucleotide analogs

A method is provided for the preparation and/or analysis or synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on strong-anion exchange HPLC columns using concentration gradients of novel 'soft base' anionic eluents, such as bromide, thiocyanate, and the like.

Method for chromatographic separation of synthetic phosphorothioate

A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.

Method of synethesizing sulfurized oligonucleotide analogs

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Redwood City, CA, United States of America

B John Bergot