Other

Taisho Pharmaceutical Co., Ltd.

Ayako Saito

Masahiko Watanabe

Takahiro Kikawada

Takashi Okuda

Yukio Sato

Reiji Kasukawa

Yasushi Kanamori

Atsushi Irisawa

Yuichi Nakahara

There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched inEST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 inoocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.

Method of increasing cell permeability to trehalose by recombinantly producing a trehalose transporter

By injecting plasmid DNAs or oligonucleotides with DNA sequence containing a 2 base sequence of unmethylated cytosine and guanine adjacent thereto into mamalian mucosal cells, mucosal immunity and CD4 positive T cells capable of producing interleukin 10 and IFN-.gamma. can be induced.

Method for inducing mucosal immunity

Growing community of inventors

Fukushima, Japan

Ayako Saito