Boehringer Mannheim Gmbh

August Wilhelm Wahlefeld

Wolfgang Gruber

Gunter Weimann

Fritz Stahler

Erich Bernt

Erich Haid

Sigmar Klose

Alexander Hagen

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##STR1## wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyl-transpeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitro-aniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

.gamma.-Glutamyl-4-nitroanilide compounds

An analytical process for enzymatic determination of a substrate in biological fluids. The fluid sample is reacted first in the absence of the specific kinase but in the presence of an NADH -- regenerating enzymatic system with the reagents needed for the reaction sequence; then the enzymes are separated from the reaction solution, and thereafter the necessary enzymes are added to the filtrate and NADH consumption is measured. The method is very accurate, economical in requiring less enzymes and only one measurement is necessary.

Analytical enzymatic determination

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyltranspeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycyl-glycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

.gamma.-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyltranspeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

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Weilheim, Germany

August Wilhelm Wahlefeld