US Government As Represented by the Secretary of the Army

Ashima Saxena

Bhupendra P Doctor

Donald M Maxwell

David E Lenz

Michelle C Ross

Alan J Russell

Richard K Gordon

Shawn R Feaster

Keith E LeJeune

Zoran Radic

Bhupandra P Doctor

Keith Lejeune

Wei Sun

Nahum Allon

Palmer Taylor

Irwin Koplovitz

Chunyuan Luo

Carolyn Chambers

Prasanthi Tipparaju

Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the HuBChE preparations.

Large-scale production of human serum butyrylcholinesterase as a bioscavenger

A novel targeting peptide from the C-terminal of endothelin and/or a novel fusogenic peptide from hemagglutinin are optionally conjugated to the carboxy group of 1,2-dioleoyl-sn-glycero-3-succinate and incorporated into liposomes for therapeutic treatment. The novel targeting peptide directs liposomes to lung cells, and, therefore, is useful for delivering liposomes encapsulating cholinesterase genes, particularly, the human serum butyryl cholinesterase (Hu BChE) gene, as a treatment against nerve agents. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader quickly to ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the appended issued claims. 37 CFR &#xa7;1.72(b).

Liposomes containing novel targeting and/or fusogenic peptides, preparations containing them and therapeutic use thereof

An enzymatically active reusable sponge or foam, capable of regeneration with oximes, made of a polymer such as polyurethane is prepared for detoxification of hazardous compounds such as organophosphorus and organosulfur compounds. The foam or sponge contains a plurality of enzymes including enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate hydrase, phosphotriesterase, paraoxonase and organophosphorus and organosulfur hydrolyzing enzymes. The sponge or foam may additionally contain activated carbon and an enzyme reactivation compound. A kit can be formed containing the sponge or foam and the compound for enzyme reactivation. The enzymatically active foam or sponge may be prepared using a two chamber device where enzymes and prepolymer are passed from separate chambers into a static mixing stator and are subjected to low shear mixing and extrusion to form by the sponge or foam.

Preparation of enzymatically active sponges or foams for detoxification of hazardous compounds

Methods, compositions and materials useful in the detection of organophosphorous and organosulfur compounds are disclosed. In particular, biosensors wherein a porous or a non-porous support having an enzyme immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.

Immobilized enzymes biosensors for chemical toxins

A method of detoxifying organophosphates using mutant cholinesterase which esists aging. The addition of oximes to the cholinesterase further retards aging of the cholinesterase.

Compositions for use to deactivate organophosphates

Growing community of inventors

Fairfax Station, VA, United States of America

Ashima Saxena