Cargill Inc.

Novozymes A/s

Ana Negrete-Raymond

Amit Vas

Holly J Jessen

Hans H Liao

Christopher K Miller

Erin Kathleen Marasco

Kenneth R Finley

Jon Veldhouse

Aristos Aristidou

Pirkko Suominen

Michael Tai Man Louie

Travis Robert Wolter

Jian Yi

Catherine B Poor

Joshua Lundorff

Brian Jeffrey Rush

Gregory Michael Poynter

Michael Glenn Catlett

Thomas W McMullin

Michelle Barnhart

Janice Frias

Briana Kozlowicz

Gui Barbier

Jonathan Dwight Veldhouse

Thomas William McMULLIN

Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO: 44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

Sugar transporter-modified yeast strains and methods for bioproduct production

Less-than-diploidcells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Genetically modified haploid

Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.

Genetically modified cells that produce C6-C10 fatty acid derivatives

A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.

Genome editing system

The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having amating factor alpha 2 (Sc MF&#x3b1;2) or repressible acid phosphatase (Sc PHO5) secretion signal.

Microorganisms expressing modified glucoamylase enzymes

Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production

Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.

Glucoamylase-modified yeast strains and methods for bioproduct production

The present application discloses the identification of the novelxylose transporter genes KHT105 and RAG4, as well as the identification of a novel set ofpentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

Compositions and methods for increased ethanol production from biomass

Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (ADC) of the Class Insecta, Bivalvia, Branchioporia, Gastropoda, or Leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.

3-hydroxypropionic acid production by recombinant yeasts expressing an insect aspartate 1-decarboxylase

Growing community of inventors

Chanhassen, MN, United States of America

Ana Negrete-Raymond