Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

Other

Boehringer Mannheim Gmbh

F. Hoffmann-la Roche Ag

Allan R Dorn

Mitali Ghoshal

Gerald F Sigler

Ronald Charles Hawley

Salvatore Joseph Salamone

John W Patterson

Erasmus Huber

Eva Hoess

Raymond Hui

Janice E Rugaber

Todd P Glancy

Catherine J Hurt

Frank E Klein

Charles F Walter

Shaker Rashid

Larry D Mountain

Charles E Durant

Joachim Siedel

Yifei Wu

A highly specific homogeneous assay method for an immunosuppressive drug using an immunophilin in a single receptor format is provided. In the simplest format, a single receptor is utilized analogous to a competitive immunoassay whereby an immunophilin is substituted for an antibody, and a competition results between a drug conjugate and the drug analyte for a limited number of immunophilin binding sites. In a microparticle agglutination assay format, an immunophilin is either bound to a particle or in solution. In the case where an immunophilin is bound to a particle, a polyvalent conjugate of the drug analyte is present in solution.

Single receptor assays for immunosuppressive drugs

The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to a method, nucleic acid molecules, vectors, and host cells for producing such a histidine-tagged, modified IMPDH polypeptide, and to kits comprising the histidine-tagged, modified IMPDH polypeptide.

Methods for producing recombinant histidine-tagged inosine nonophosphate dehydrogenase polypeptides

The present invention provides a method for the measurement of an analyte in biological samples whereby an uncompetitive inhibitor is coupled to a ligand and utilized in a homogeneous assay. The analyte can be a drug or drug derivative, hormone, polypeptide, or oligonucleotide. The present invention also provides novel compounds, assay reagents and packaged kits useful for performing such measurements.

Conjugates of mycophenolic acid

Recombinant histidine-tagged inosine monophosphate dehydrogenase polypeptides

Conjugates of uncompetitive inhibitors of inosine monophosphate dehydrogenase

Enzyme inhibition immunoassay

The present invention provides a method for the enzymatic measurement of mycophenolic acid and other IMPDH inhibitors in biological samples. The present invention also provides assay reagents and packaged kits useful for performing enzymatic measurement of mycophenolic acid and other IMPDH inhibitors in biological samples.

Enzymatic measurement of mycophenolic acid

The invention provides sets of liquid reagents that afford calibration stability in an enzyme based spectrophotometric assay for the measurement of lactate in patient samples. The reagent sets include a lactate oxidase, a peroxidase, a hydrogen donor, an agent that substantially prevents ascorbic acid interference, and agent that substantially prevents bilirubin interference, a coupling agent, a buffer and, optionally, a preservative. The invention further provides methods for using the liquid reagent sets.

Liquid reagent set for L-lactate determination

Novel derivatives of procainamide and N-acetylprocainamide (NAPA) are disclosed having the following formula: ##STR1## wherein: X=hydrogen or acetyl;

Maleimide adduct conjugates of procainamide and NAPA

Cell separation process

Cell separation compositions and associated methods effective for separating cells from various biological specimens such as blood are disclosed. The biological specimens are contacted with the cell separation compositions and centrifuged. The cells are separated based upon their buoyant density in the cell separation composition. The cell separation composition contains colloidal silica particles covalently linked to an organosilane having a non-ionic, hydrophilic group.

Reagent for cell separation

Growing community of inventors

Carmel, IN, United States of America

Allan R Dorn