Praxis Biologics, Inc.

American Cyanamid Company

Clariant Finance (bvi) Limited

Algis Anilionis

Gary Warren Zlotnick

Bruce Arthur Green

Robert A Deich

Robert C Seid, Jr

Robert N Brey, Iii

James P Fulginiti

Roberta L Farrell

Kashayar Javaherian

Jennifer A Jackson

Theodore E Maione

Paul Gelep

Bruce A Sadownick

James Rusche

Robert C Seid

This invention pertains to a complementation system for the selection and maintenance of expressed genes in bacterial hosts. The invention provides stable vectors which can be selected and maintained by complementation of chromosomal deletion mutations of purA (adenylosuccinate synthetase), obviating the use of antibiotic resistance genes. This system is useful in production organisms during fermentation and in live vaccine bacteria, such as attenuated Salmonella typhi. This system allows for selection of chromosomal integrants and for selection and stable plasmid maintenance in the vaccinated host without application of external selection pressure.

Stable pura vectors and uses therefor

Stable purA vectors and uses therefor

The gene encoding the ligninase rLDM.TM. 6 has been cloned and expressed. The ligninases produced via the processes disclosed herein are useful to degrade or modify lignin in pulp operations.

Cloning and expression of ligninases

Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods including novel and improved methods of purification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequences encoding PBOMP-1 and PBOMP-2 related peptides, proteins and fusion proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides, proteins, and fusion proteins in appropriate host cells. The peptides, proteins, fusion proteins and viruses both 'live' and 'inactivated' are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides, proteins and fusion proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides, proteins and fusion proteins in hybridization assays is also described.

Recombinant vectors for Haemophilus influenzae peptides and proteins

Vaccines for Haemophilus influenzae

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Pittsford, NY, United States of America

Algis Anilionis