Medimmune Limited

Medlmmune Limited

Alan Hunter

Christopher D Thompson

Thomas Linke

Ambarish Shah

William K Wang

Hasige Sathish

Timothy Pabst

Xiangyang Wang

Liu Tie

Michaela Wendeler

Yiming Li

Mariko Fonseca

Jihong Wang

Robert Strouse

Guoling Xi

Andrew Fulton

Kendall Carey

Johnson Varghese

Tim Pabst

Chris Thompson

The present invention relates to a method of purifying albumin-fusion proteins to reduce the level of oxidation of susceptible amino acid residues. The method comprises an affinity matrix chromatography step and an anion exchange chromatography step. The purified albumin-fusion proteins have low levels of oxidation and retain their enhanced half-life in vivo and its bioactivity. In some embodiments, the albumin-fusion protein comprises a scaffold, such as human Tenascin C scaffold. Compositions comprising the albumin-fusion protein are further disclosed.

Method of purifying albumin-fusion proteins

The present invention provides methods for isolating an active polypeptide or immunoconjugate by purification of a solution containing both the active polypeptide or immunoconjugate and an acidic variant thereof, such as a deamidated variant, using anion exchange chromatography. The present invention also provides compositions, formulations, and unit dosage forms comprising the purified polypeptide or immunoconjugate.

Method for purifying active polypeptides or immunoconjugates

The present invention provides methods of preparing active immunoconjugates, including anti-CD22 immunoconjugates. Suitably, the methods include a fed-batch refolding process and/or column stripping process that result in an increase in yield of the immunoconjugate over other processes that do not utilize the methods.

Methods of producing immunoconjugates

The present application relates to methods for purifying recombinant coagulation factor proteins, including for example, prothrombin (Factor II). In embodiments, the methods provide purified prothrombin that exhibit increased bioactivity and reduced levels of thrombin, thereby increasing the safety of the prothrombin. Also provided are purified recombinant coagulation factor proteins.

Methods for processing coagulation factors

The present invention provides methods for isolating an active polypeptide or immunoconjugate by purification of a solution containing both the active polypeptide or immunoconjugate and an acidic variant thereof, such as a deamidated variant, using anion exchange chromatography.

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Gaithersburg, MD, United States of America

Alan Hunter