Juridical Foundation the Chemo-sero-therapeutic Research Institute

Science and Technology Agency, Minister's Secretariat Director of

Akio Toh-e

Atsushi Miyanohara

Kenichi Matsubara

Chikateru Nozaki

Fukusaburo Hamada

Nobuya Ohtomo

A shuttle vector containing an yeast gene and an E. coli gene and carrying the expression control region of acid phosphatase gene of yeast, which can be recombined with various genes under control of the phosphatase promoter, to give various recombinant plasmids. The shuttle vector is useful in genetic engineering industries.

Shuttle vector

A recombinant plasmid inserted with Hepatitis B virus gene, which comprises a plasmid vector containing a yeast gene and an E. coli gene and carrying the expression control region of the repressible acid phosphatase gene of yeast and a Hepatitis B virus gene recombined thereto under control of the phosphatase promoter, a transformed yeast which is prepared by transforming a yeast with the recombinant plasmid, and a method of the production of Hepatitis B virus surface antigen in a large scale by culturing the transformed yeast in a medium. The Hepatitis B virus surface antigen prepared by the present invention has the same immunological properties as those of the natural antigen from human blood plasma and is useful for the preparation of Hepatitis B virus vaccine and diagnostic reagents.

Recombinant plasmid inserted with hepatitis B virus gene, yeast

Growing community of inventors

Hiroshima, Japan

Akio Toh-e