Shionogi Company Limited

Hokkaido University

Akio Takimoto

Takayasu Sonoyama

Tomoaki Takakura

Kenji Mitsushima

Shin-Ichiro Nishimura

Shigeo Yagi

Yoshiaki Miura

Yasuro Shinohara

Kenji Inagaki

Jun-ichi Furukawa

Hidehiko Tanaka

Yoko Kita

Mika Nakano

Kensaku Akita

Shigeo Yaqi

Takaomi Ito

Shintaro Misaki

Hirofumi Nagatome

Takaomi c/o Shionogi Innovation Center For Drug Discovery Ito

A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention. A salt of the general formula (I): wherein Z, X, R, R, M, m and n are the same as described in DESCRIPTION,is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.

Method of serum pre-treatment for glycomic analysis

An L-methionine &#x3b3;-lyase having modified function which is obtained by structurally stabilizing natural L-methionine &#x3b3;-lyase by analyzing the structure of crystals thereof by using X-ray, estimating an amino acid sequence concerning its substrate specificity and varying amino acid residue thereof; enzymes comprising a protein constituting the L-methionine &#x3b3;-lyase having modified function or its multimer (preferably its tetramer); DNA sequences encoding these enzymes; a process for producing these enzymes; and medicinal preparations (preferably anticancer agent) containing these enzymes.

L-methionine $g(g)-lyase with modified function

A process for production of L-methionine &ggr;-lyase crystals by using polyethylene glycol, characterized by comprising the first step of warming a solution containing L-methionine &ggr;-lyase before or after addition of polyethylene glycol thereto and the second step of adding an inorganic salt.

Process for producing L-methionine &ggr;-lyase crystals

A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transfomed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.

Cephalosporin acetylhydrolase gene and protein encoded by said gene

A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.

Cephalosporin acetylhydrolase gene from Bacillus subtilis

Growing community of inventors

Amagasaki, Japan

Akio Takimoto