Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Akio Ozaki

Katsuhiko Ando

Keiko Ochiai

Hideo Mori

Satoshi Koizumi

Kazuhiko Tabata

Tetsuo Endo

Shigeru Chiba

Takeshi Shibasaki

Hiroaki Motoyama

Koichiro Miyake

Shinichi Hashimoto

Ryoichi Katsumata

Shin-ichi Hashimoto

Haruo Seto

Yoshiyuki Yonetani

Tetsuo Oka

Yoichi Uosaki

Tomohisa Kuzuyama

Shunji Takahashi

Hiroshi Mizoguchi

Akira Furuya

Katsutoshi Sasaki

Hirofumi Endo

Isao Kawamoto

Yukio Hashimoto

Haruhiko Yokoi

Hideki Kawasaki

Yutaka Takano

Masaru Hasegawa

Keishiro Tamura

Satoshi Nakagawa

Masato Ikeda

Seiga Itoh

Tamio Mizukami

Hiroshi Nagano

Satoru Furukawa

Toshihide Nakanishi

Toru Mizukami

Mikiro Hayashi

Junko Ohnishi

Yukinobu Kotani

Masahiro Sugimoto

Seiko Ando

Motoko Kageyama

Morimasa Yagisawa

Naoko Tateishi

Akihiro Senoh

Shinichi Lsp Machida 1203 Hashimoto

Shunji Greenhouse B 102 Takahashi

This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

Processes for producing sugar nucleotides and complex carbohydrates

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,

Process for producing HMG-CoA reductase inhibitor

The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

Process for producing N-acetylneuraminic acid

The present invention provides a process for producing isoprenoid compounds or proteins encoded by DNA using DNA that contains one or more of the DNA encoding proteins having activity to improve efficiency in the biosynthesis of isoprenoid compounds effective in pharmaceuticals for cardiac diseases, osteoporosis, homeostasis, prevention of cancer, and immunopotentiation, health food and anti-fouling paint products against barnacles; the DNA; the protein; and a method for screening a substance with antibiotic and weeding activities comprising screening a substance inhibiting enzymatic reaction on the non-mevalonate pathway.

Process for producing isoprenoid compounds by microorganisms and a method for screening compounds with antibiotic or weeding activity

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): 

DNA encoding hydroxylase

The present invention provides a process for producing ubiquinone-10 using a microorganism having the ability to form ubiquinone-10 and having one or more properties selected from the group consisting of the property wherein geranylgeranyltransferase activity is reduced or defective, the property wherein decaprenyldiphosphate synthetase activity is strengthened, and the property wherein p-hydroxybenzoic acid-decaprenyltransferase activity is strengthened, DNA and a polypeptide useful for the production process, microorganisms useful for the production, a method for expressing a gene in the microorganisms, and a method for breeding the microorganisms.

[object Object]

Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genusorand which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.

Process for producing trans-4-hydroxy-L-proline

An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

Process for producing cis-3-hydroxy-L-proline

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,or a ring-closed lactone form thereof;a DNA encoding the protein; and a recombinant DNA comprising the DNA.

The present invention provides a novel protein having N-acetylglucosamine 2-epimerase activity; a DNA encoding the protein; a recombinant vector containing the DNA; a transformant obtainable by introducing the recombinant vector into a host cell; and a process for producing the protein or N-acetylmannosamine using the transformant.

N-acetylglucosamine 2-epimerase and DNA encoding the same

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Machida, Japan

Akio Ozaki