Adelaide Research & Innovation Pty Ltd

Griffith University

The University of Adelaide

Sibtech, Inc.

Adelaide Research and Innovation

Adrienne Webster Paton

James Cleland Paton

Michael Paul Jennings

Marina V Backer

Joseph M Backer

Christopher Day

A mutant subtilase cytotoxin B subunit protein is provided which can bind glycans having &#x3b1;2-3-linked N-glycolylneuraminic acid and glycans having &#x3b1;2-6-linked N-glycolylneuraminic acid. The mutant SubB protein has deletions of one or more of the amino acid sequence TTSTE and has a previously undescribed ability to bind glycans having &#x3b1;2-6-linked N-glycolylneuraminic acid, while not losing the ability to bind glycans having &#x3b1;2-3-linked N-glycolylneuraminic acid.

Subtilase cytotoxin B subunit mutant

A member of a new class of bacterial toxin has been isolated and characterised. The bacterial toxin is of the ABtype and is characterised in that it has a subtilase domain. It is cytotoxic to Vero cells, and toxicity in vivo in mice occurs in a number of different sites. Mutation of the serine active residue results in greater than 99% reduction in activity. The protein has been purified and antibodies have been prepared for both the A subunit and B subunits, and ELISA detection methods have been developed. The nucleic acid sequence has been determined and primers specific for the toxin have been used for a preliminary screen of a range of patient samples to ascertain the extent to which the toxin is present.

Cytotoxin with a subtilase domain

Growing community of inventors

Parkside, Australia

Adrienne Webster Paton