Arizona State University

National Technology & Engineering Solutions of Sandia, LLC

Aditya Pandharinath Sarnaik

Ryan Wesley Davis

Joseph S Schoeniger

Arul Mozhy Varman

Muhammad Faisal

Amit Kumar Jha

Arul Mohzy Varman

Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters.was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate.

Production of fusel lactates via biocatalysis

A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.

Engineering RuBisCo for food safety

Growing community of inventors

Tempe, AZ, United States of America

Aditya Pandharinath Sarnaik