The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 12, 2022
Filed:
Nov. 07, 2019
Applicant:
Agilent Technologies, Inc., Santa Clara, CA (US);
Inventors:
Bahram Arezi, Carlsbad, CA (US);
Michael Borns, Escondido, CA (US);
Holly Hogrefe, San Diego, CA (US);
Connie Hansen, San Diego, CA (US);
Assignee:
Agilent Technologies, Inc., Santa Clara, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2005.12); C12Q 1/6806 (2017.12); C12Q 1/6874 (2017.12); C12Q 1/6869 (2017.12);
U.S. Cl.
CPC ...
C12Q 1/6874 (2012.12); C12Q 1/6806 (2012.12); C12Q 1/6869 (2012.12);
Abstract
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.