The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 06, 2009
Filed:
Aug. 11, 1993
Shan-rong Shi, Los Angeles, CA (US);
Atul K. Tandon, Fremont, CA (US);
Krishan L. Kalra, Danville, CA (US);
Nagesh Malhotra, Los Angeles, CA (US);
Sheng-hui Su, San Ramon, CA (US);
Cheng-zhi Yu, Pleasant Hill, CA (US);
Shan-Rong Shi, Los Angeles, CA (US);
Atul K. Tandon, Fremont, CA (US);
Krishan L. Kalra, Danville, CA (US);
Nagesh Malhotra, Los Angeles, CA (US);
Sheng-Hui Su, San Ramon, CA (US);
Cheng-Zhi Yu, Pleasant Hill, CA (US);
BioGenx Laboratories, Inc., San Ramon, CA (US);
Abstract
A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.