The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 27, 2008
Filed:
Oct. 07, 1997
Jan Hendrick Houthoff, Amsterdam, NL;
Jan Reedijk, Leiden, NL;
Tinka Jelsma, Almere, NL;
Robert Jochem Heetebrij, Leiden, NL;
Herman Hendricus Volkers, Monnickendam, NL;
Jan Hendrick Houthoff, Amsterdam, NL;
Jan Reedijk, Leiden, NL;
Tinka Jelsma, Almere, NL;
Robert Jochem Heetebrij, Leiden, NL;
Herman Hendricus Volkers, Monnickendam, NL;
Kreatech Biotechnology B.V., Amsterdam, NL;
Abstract
The present invention provides improved methods for labeling nucleotides. The method of the invention for labeling nucleotides comprises the steps of: reacting a reactive moiety of a linker, which linker is a platinum compound having a stabilizing bridge and two reactive moieties, with an electron donating moiety of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label; reacting the other reactive moiety of said linker with a nucleotide. A major advantage of the invention is that all nucleotides can be labeled by the method of the invention, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.