The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 1994
Filed:
Sep. 29, 1991
Frederick C Baldauf, Bel Air, MD (US);
Brent R Busey, Bel Air, MD (US);
Kenneth E Thames, Aiken, SC (US);
The United States of America as represented by the Secretary of the Army, Washington, DC (US);
Abstract
A method is provided for the integration of a low-volume liquid flow and ing system and a portable optical waveguide-based fluorescence detector for the chemical analysis of reagents in fluorescence-based reactions. The method is particularly applicable to the detection of enzyme inhibitors, modifiers, or ligands in cases where a fluorescent enzyme substrate or product exists. In a preferred embodiment, the presence and concentration of acetylcholinesterase inhibitors, such as chemical warfare nerve agents or certain insecticides, is determined by mixing aqueous samples with a dilute solution of n-methyl indoxyl acetate, and monitoring the formation of a fluorescent product (n-methyl indoxyl). The presence of an acetylcholinesterase inhibitor reduces the amount of fluorescent product formed in a given time period. The reaction product passes through a flow cell in which it is excited by the evanescent wave produced by light as the light undergoes total internal reflection within an optical rod or fiber, or by light coming out the end-face of an optical fiber or rod.